Section 1: Engineering Mathematics

Linear Algebra: Matrices and determinants, Systems of linear equations, Eigen values and Eigen  vectors.  Calculus: Limit, continuity  and  differentiability,  Partial  derivatives,  Maxima and  minima,  Sequences  and  series,  Test  for  convergence,  Fourier  Series.  Differential Equations:   Linear   and   nonlinear   first   order   ODEs,   higher   order   ODEs   with   constant coefficients, Cauchy’s and Euler’s equations, Laplace transforms, PDE-Laplace, heat and wave equations. Probability and Statistics: Mean, median, mode and standard deviation, Random variables, Poisson, normal and binomial distributions, Correlation and regression analysis.   Numerical   Methods:   Solution   of   linear   and   nonlinear   algebraic   equations, Integration of trapezoidal and Simpson’s rule, Single and multistep methods for differential equations.

Section 2: General Biotechnology

Biochemistry:   Biomolecules-structure   and   functions;   Biological   membranes,   structure, action potential and transport processes; Enzymes- classification, kinetics and mechanism of action; Basic concepts and designs of metabolism (carbohydrates, lipids, amino acids and nucleic acids) photosynthesis, respiration and electron transport chain; Bioenergetics

Microbiology:    Viruses-    structure    and    classification;    Microbial    classification    and diversity(bacterial,  algal  and  fungal)Methods  in  microbiologyMicrobial  growth  and nutritionAerobic  and  anaerobic  respirationNitrogen  fixationMicrobial  diseases  and host-pathogen interaction

Cell Biology: Prokaryotic and eukaryotic cell structure; Cell cycle and cell growth control;

Cell-Cell communication, Cell signaling and signal transduction

Molecular   Biology   and   Genetics:   Molecular   structure   of   genes   and   chromosomes; Mutations  and  mutagenesis; Nucleic  acid  replication,  transcription,  translation  and  their regulatory  mechanisms  in  prokaryotes  and  eukaryotes;  Mendelian  inheritance;  Gene interaction; Complementation; Linkage, recombination and chromosome mapping; Extra chromosomal  inheritance;  Microbial   genetics  (plasmids,  transformation,  transduction, conjugation); Horizontal gene transfer and Transposable elements; RNA interference; DNA damage and repair; Chromosomal variation; Molecular basis of genetic diseases Analytical Techniques: Principles of microscopy-light, electron, fluorescent and confocal; Centrifugation-  high  speed  and  ultra;  Principles  of  spectroscopy-UV,  visible, CD,  IR, FTIR, Raman, MS,NMR; Principles of chromatography- ion exchange, gel filtration, hydrophobic interaction, affinity, GC,HPLC, FPLC; Electrophoresis; Microarray

Immunology:  History  of  Immunology;  Innate,  humoral  and  cell  mediated     immunity; Antigen;  Antibody  structure and function; Molecular basis of antibody diversity; Synthesis of   antibody   and   secretion;   Antigen-antibody   reaction;   Complement;   Primary   and secondary  lymphoid  organ;  B  and  T  cells  and  macrophages;  Major  histocompatibility complex   (MHC);   Antigen   processing   and   presentation;   Polyclonal   and   monoclonal antibody;    Regulation    of    immune    response;    Immune    tolerance;    Hypersensitivity; Autoimmunity; Graft versus host reaction.

Bioinformatics:  Major  bioinformatic  resources  and  search  tools;  Sequence  and  structure databasesSequence  analysis  (biomolecular  sequence  file  formats,  scoring  matrices, sequence  alignment,  phylogeny);Data  mining  and  analytical  tools  for  genomic  and

proteomic studies; Molecular dynamics and simulations  (basic concepts including force fields, protein-protein, protein-nucleic acid, protein-ligand interaction)

Section 3: Recombinant DNA Technology

Restriction  and  modification  enzymes;  Vectors;  plasmid,  bacteriophage  and  other  viral vectors,   cosmids,   Ti   plasmid,   yeast   artificial   chromosome;   mammalian   and   plant expression   vectors;   cDNA   and   genomic   DNA   library;   Gene   isolation,   cloning   and expression ; Transposons and gene targeting; DNA labeling; DNA sequencing; Polymerase chain  reactions;  DNA  fingerprinting;  Southern  and  northern  blotting;  In-situ  hybridization; RAPD, RFLP; Site-directed mutagenesis; Gene transfer technologies; Gene therapy

Section 4: Plant and Animal Biotechnology

Totipotency; Regeneration of plants; Plant growth regulators and elicitors; Tissue culture and   Cell   suspension   culture   system:   methodology,   kinetics   of   growth   and,   nutrient optimization; Production of secondary metabolites by plant suspension cultures; Hairy root culture; transgenic plants; Plant products of industrial importance.

Animal  cell  culture;  media  composition  and  growth  conditions;  Animal  cell  and  tissue preservation;  Anchorage  and  non-anchorage  dependent  cell  culture;  Kinetics  of  cell growth;  Micro  &  macro-carrier  culture;  Hybridoma  technology;  Stem  cell  technology; Animal cloning; Transgenic animals

Section 5: Bioprocess Engineering and Process Biotechnology

Chemical engineering principles applied to biological system, Principle of reactor design, ideal   and   non-ideal   multiphase   bioreactors,   mass   and   heat   transfer;   Rheology   of fermentation fluids, Aeration and agitation; Media formulation and optimization; Kinetics of  microbial  growth,  substrate  utilization  and  product  formation;  Sterilization  of  air  and media;  Batch,  fed-batch  and  continuous  processes;  Various  types  of  microbial  and enzyme reactors; Instrumentation control  and optimization; Unit operations in solid-liquid separation   and   liquid-liquid   extraction;   Process   scale-up,   economics   and   feasibility analysis

Engineering    principle    of    bioprocessing-    Upstream    production    and    downstream; Bioprocess  design  and  development  from  lab  to  industrial  scale;  Microbial,  animal  and plant cell  culture  platforms;  Production  of  biomass  and  primary/secondary  metabolites; Biofuels,    Bioplastics,    industrial    enzymes,    antibiotics;    Large    scale    production    and purification  of  recombinant  proteins;  Industrial  application  of  chromatographic  and membrane  based  bioseparation  methods;  Immobilization  of  biocatalysts  (enzymes  and cells) for  bioconversion  processes; Bioremediation-Aerobic  and  anaerobic  processes  for stabilization of solid / liquid wastes.